Figure 3. Total Cytochrome P450 Content in Primary Porcine Hepatocytes Cultured in Four Separate Chemically-Defined Media Formulations.
Total cytochrome P450 content of porcine hepatocytes cultured at 107 viable cells per 100 mm dish in WE, ML-15, M199 and M1640 chemically-defined media for up to 6 days. tCYP450 content in freshly isolated cells and cell cultures scraped from dishes on days 2, 4 and 6 was determined by carbon monoxide difference spectroscopy of the sodium hydrosulphite reduced samples as described above. Results are expressed as pmol/mg total protein. All values represent the means ± SEM of three experiments in duplicate. Statistics: Day 4: WE vs ML-15, p = 0.043; ML-15 vs M1640, p = 0.047. Day 6: WE vs M199, p = 0.026; WE vs M1640, p = 0.033.