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Effect of MTX concentration on cellular MTX polyglutamate distribution. Following a 24-hour exposure to MTX at concentrations of 10, 100, and 1000 nM, the percentage of total cellular MTX represented by the parent drug (i.e., MTX-PG1), each of its polyglutamate metabolites (i.e., MTX-PG2 through MTX-PG6), and the sum total of the polyglutamate metabolites (MTX-PG2-6) was determined. Each sample is presented as the mean ± S.D. from three independent experimental evaluations.
