TABLE 1.

Sequences of the probes and primers used in the study

Name	Direction	Sequence
EMSA probes
GS-ECR	Forward	5′-gatcCTTAGTTATTCATTTCTGCAGAACTAATTTTTAACCTAG-3′
GS-ECR	Reverse	5′gatcCTAGGTTAAAAATTAGTTCTGCAGAAATGAATAACTAAG-3′
GS-ECR_mut	Forward	5′-gatcCTTA-GGGGTTCAGGTCTGCAGAACGGATTTTGGGCCTAG-3′
GS-ECR_mut	Reverse	5′-gatcCTAGGCCCAAAATCCGTTCTGCAGACCTGAACCCCTAAG-3′
GS-HNF1_cons	Forward	5′-gatcCCAGGTTAATGATTAACCCA-3′
GS-HNF1_cons	Reverse	5′-gatcTGGGTTAATCATTAACCTGG-3′
ChIP primers
ChIP-ECR	Forward	5′-CTGGCTCGTGGGTAAGAATTGTCTC-3′
ChIP-ECR	Reverse	5′ATGTCCAAGGCAATGCTAGGTTAAA-3′
ChIP-Intron2	Forward	5′-GGCAGCGAGATCGAAGGACAACTGT-3′
ChIP-Intron2	Reverse	5′-TCTCCTGCCTTCGGGTTTTCTCCAA-3′
Primers used in the generation of the reporter gene and the HNF1-overexpressing constructs
OCT1 Promoter	Forward	5′-TGCACAGAGAGAGAAACCAAAAGTC-3′
OCT1 Promoter	Reverse	5′-GCCAGCTCGAGATGTCTCCCTCAGAGATCTTTG-3′
ECR	Forward	5′-TCCTTGGATCCCCAGCTCCTCCTCCAAACT-3′
ECR	Reverse	5′-CCCGAGTCGACCTCATGATCTTAGCACCTAGCCTT-3′
HNF1A	Forward	5′-CCTGTGGATCCGAGCCATGGTTTCTAAACTGA-3′
HNF1A	Reverse	5′-AGCTTATCTAGAGTGGTTACTGGGAGGAAGAGG-3′
HNF1B	Forward	5′- CTTTTTCCGGATCCTTGGAAAATGGTGTCCAA-3′
HNF1B	Reverse	5′- GTGGTGTCTAGAGGCATCACCAGGCTTGTAGA-3′
Primers used for site-directed mutagenesis of the ECR1 Binding Sites
HNF1A(A)_f	Forward	5′-TTGGGGAATCAATCTTAGGGGTTCAGGTCTGCAGAACTAATTTTT A-3′
HNF1A(A)_r	Reverse	5′-TAAAAATTAGTTCTGCAGACCTGAACCCCTAAGATTGATTCCCCAA-3′
HNF1A(B)_f	Forward	5′-GGTTCAGGTCTGCAGAACGGATTTTGGGCCTAGCATTGCCTTGGAC-3′
HNF1A(B)_r	Reverse	5′-GTCCAAGGCAATGCTAGGCCCAAAATCCGTTCTGCAGACCTGAACC-3′

ChIP, chromatin immunoprecipitation. The unspecific sequences used in the radioactive labeling of the EMSA probes are given in lowercase letters. Mutated nucleotides are shown in boldface, and the artificially introduced restriction sites are underlined. The ATG start codons of HNF1α and HNF1β are given in italics.