Fig. 11.

Peptide #1 and MK-5046 stimulate [³H]arachidonic acid release in hBRS-3 Balb 3T3 transfected cells. The hBRS-3 Balb cells (5 × 10⁴ cells/ml) were incubated with [³H-5,6,8,9,11,12,14,15]arachidonic acid (1 μCi/4 ml, [³H]AA) for 24 hours at 37°C, washed, and incubated with the indicated unlabeled concentration of MK-5046 or peptide #1 for 40 minutes at 37°C. [³H]AA release was determined as described under Materials and Methods. The results are expressed as the percentage of the release seen with a maximally effective concentration of peptide #1 (1 μM) and are the mean ± S.E.M. from at least four experiments. In each experiment, the data points were determined in duplicate. The control and the maximal stimulated (1 μM peptide #1) values were 901 ± 94 and 1542 ± 113 dpm, respectively (n = 5).