TABLE 3.
Effect of various inhibitors on the ability of peptide #1 to stimulate phospholipase A2 and release [3H]arachidonic acid in hBRS Balb 3T3 cells
Cells were incubated with 1 μCi/4 ml of [3H-5,6,8,9,11,12,14,15]arachidonic acid for 24 hours, then washed and incubated with the indicated unlabeled compound for 40 minutes at 37°C. [3H]AA release was determined as described under Materials and Methods. Results are mean ± S.E.M. from at least four experiments.
| Additions |
[3H]Arachidonic Acid Released |
|---|---|
| dpm | |
| None | 366 ± 41 |
| Peptide #1 alone (0.1 μM) | 874 ± 121a |
| + Bantag-1 (1 μM) | 432 ± 56b |
| + Manoalide (1 μM) | 553 ± 44b |
| + Quinacrine (1 μM) | 614 ± 32b |
| + AACOCF3 (1 μM) | 574 ± 63b |
| + U-73122 (1 μM) | 950 ± 123 |
| + RHC-80267 (1 μM) | 1067 ± 224 |
a
P < 0.01 compared with no additions.
b
P < 0.005 compared with peptide #1 alone.