Skip to main content
. 2013 Oct;347(1):38–46. doi: 10.1124/jpet.113.207647

Fig. 2.

Fig. 2.

UDP, UDP-sugars, and LTC4 do not promote accumulation of inositol phosphates in COS-7 or HEK293 cells transiently coexpressing GPR17 and Gαq/i5. Application of UDP, UDP-glucose (UDP-Glu), UDP-galactose (UDP-Gal) (all at 100 μM), and LTC4 (100 nM) all failed to promote significant inositol phosphate accumulation in (A) COS-7 or (B) HEK293 cells transfected with GPR17 alone or cotransfected with GPR17 and Gαq/i5, which links Gi/o-coupled receptors to the PLC signaling pathway. In contrast, UDP, UDP-Glu, UDP-Gal, and LTC4 promoted robust accumulation of inositol phosphates in cells expressing their cognate receptors (UDP at P2Y6 receptors, UDP-Glu and UDP-Gal at P2Y14 receptors, and LTC4 at CysLTR1 receptors). (C) Cell surface expression of HA-tagged GPR17 was verified by an intact cell RIA. Data shown are representative assays performed three times with similar results. cpm, counts per minute.