Fig. 5.

Lack of uracil nucleotide– and leukotriene-promoted intracellular Ca²⁺ mobilization in 1321N1-GPR17 cells. GPR17-expressing 1321N1 cells were grown on glass coverslips to a density of 20% of confluence. Cells were placed in a sealed chamber and continuously superfused with HBSS with or without (A) 100 μM UDP, (B) 100 μM UDP-glucose (UDP-Glu), (C) 100 μM UDP-galactose (UDP-Gal), (D) 100 nM LTC4, or (A–D) 1 mM carbachol (Carb). Each compound was superfused for 1 minute. Traces of intracellular Ca²⁺ concentrations were averaged from 6–9 individual cells/coverslip. Each compound was tested on a minimum of three coverslips.