Fig. 6.

Neither UDP, UDP-glucose (UDP-G), nor LTC4 promotes internalization of GPR17 stably expressed in 1321N1 cells. 1321N1 cells stably expressing CysLTR1, P2Y₂, or GPR17 receptors were seeded on glass coverslips for 2 days, then cooled down to 4°C and labeled with mouse monoclonal anti-HA.11 antibody for 1 hour. Cells were washed, warmed to 37°C, and treated with either phosphate-buffered saline (PBS) or receptor agonists (100 μM UTP, UDP, or UDP-G or 100 nM LTC4) for 30 minutes. The cells were then fixed, permeabilized, and stained with goat anti-mouse A-488 secondary antibody. Localization of receptors was determined by confocal microscopy.