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GPR17 negatively regulates CysLTR1 function. 1321N1 cells stably expressing 3× HA-tagged CysLTR1 were infected with retroviruses directing expression of myc-tagged GPR17, and the resulting cells were assessed for LTC4-promoted inositol phosphate accumulation (A) and CysLTR1 expression by a cell surface RIA (B). Data shown are from a representative assay performed three times with similar results. Ab, antibody; cpm, counts per minute.
