Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 17;62(10):3459–3468. doi: 10.2337/db12-1833

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

PMC Copyright notice

FIG. 3. — Duct-specific Pdx1-deficient mice had impaired glucose tolerance and impaired insulin secretion. A: Time course of morning fed blood glucose values of the controls (solid line, n = 33), CAII^Cre;Pdx1^Fl^/^Fl (dashed line, n = 17), and CAII^Cre;Pdx1^Fl^/⁺ (dotted line, n = 23) littermates. Only at 3 and 4 weeks did the two bigenic genotypes differ from each other. B: Intraperitoneal glucose tolerance test (IPGTT) in 10-week-old animals comparing control (solid line) and bigenic mice (CAII^Cre;Pdx1^Fl^/^Fl and CAII^Cre;Pdx1^Fl^/⁺, dashed-dotted line; n = 8–16) showed impaired glucose tolerance. C: Plasma insulin levels from the IPGTT showed significant increases in both groups at 15 min after glucose injection compared with fasting at 0 min in controls (□, n = 4) and bigenic (■, n = 9). *P < 0.025 compared with 0 min. #P < 0.004 comparing groups at 15 min. D–E: Isolated islets from 11-week-old bigenic mice (both CAII^Cre;Pdx1^Fl^/^Fl and CAII^Cre;Pdx1^Fl^/⁺, ■, n = 10 animals) in sequential static incubation had impaired glucose-responsive insulin secretion compared with controls (□, n = 10 animals) (D) and lower percentage insulin content secreted (E) even though the islet insulin content was not significantly different (F). Data are mean ± SEM. *P < 0.007. Even if each islet aliquot with values for both glucose concentrations (n = 23 for bigenic and n = 26 for control) was used for the averaging, the basal levels and islet insulin content do not differ, but the bigenic islets showed a modest glucose-stimulated insulin release (2.6 mmol/L glucose: 3.6 ± 1.1 pg insulin/ng DNA; 16.8 mmol/L glucose: 12.5 ± 3.6 pg insulin/ng DNA; P < 0.003, paired t test).