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. 2013 Sep 17;62(10):3350–3361. doi: 10.2337/db13-0500

FIG. 1.

FIG. 1.

Fiber type–specific expression of endogenous ATGL in murine skeletal muscle and regulation by HFD feeding. ATGL protein expression (ATGL IF in 2B fibers of GAKO mice set to 0) (A) and IMTG content by ORO staining (ORO IF of 2B fibers of WT mice set to 1) (B) in skeletal muscle fibers of chow-fed WT versus GAKO mice (♀, chow, 10 weeks, C57BL/6, gastrocnemius-plantaris-soleus [GPS] complex; n = 3 to 4/group). ATGL protein expression (ATGL IF in 2B fibers of WT-chow mice set to 1) (C) and IMTG content by ORO staining (ORO IF in 2B fibers of WT-chow mice set to 1) (D) in skeletal muscle fibers of chow- versus HFD-fed WT mice (♀, 22 weeks FVB, GPS complex; n = 3 to 4/group). E: Relationship between ATGL and IMTG (data from C and D). F: Representative images of ATGL and ORO IF in chow- and HFD-fed mice demonstrating overlap and enhanced staining with HFD. For overall effects having P < 0.05: D, diet; F, fiber type; G, genotype. For specific comparisons having P < 0.05: #for effect of diet; *for effect of genotype. AU, arbitrary units.