FIG. 5.

Chronic administration of IGF-I and AspB10 led to increased phosphorylation of Akt, but no change in Erk1/2 phosphorylation. Western blot analysis of MVT1 tumor lysates at the end of the treatment periods with rhIGF-I and AspB10 revealed that rhIGF-I and AspB10 treatment led to increased Akt phosphorylation and no change in Erk1/2 phosphorylation (A–D). Graphs are representative of two studies for AspB10 treatment and three studies for rhIGF-I treatment. All graphs show the mean for each group, and error bars represent the SEM. Statistical analysis was performed using two-tailed t test. *P < 0.05 between groups. n = 9–11 mice per group. p, phosphorylated; MKR V, MKR mice with endogenous hyperinsulinemia.