Skip to main content
. Author manuscript; available in PMC: 2014 Aug 8.
Published in final edited form as: Mol Cell. 2013 Aug 8;51(3):326–337. doi: 10.1016/j.molcel.2013.07.008

Figure 5. MutS and MutL confer directionality to UvrD-mediated unwinding of homeologous recombination intermediates.

Figure 5

(A) Resolution of homeologous strand exchange intermediates by UvrD is bidirectional, but becomes unidirectional when guided by mismatch-activated MutS-MutL complexes. From left to right, homeologous strand exchange without MMR proteins, with UvrD (1 nM), with MutS-MutL (75, 75 nM) and MutS-MutL-UvrD (75, 75, 1 nM). See also Figures S4, and S5.

(B) MutS, MutL and UvrD do not resolve homologous strand exchange intermediates. From left to right, homologous strand exchange without MMR proteins, with UvrD (1 nM), with MutS-MutL (75, 75 nM) and MutS-MutL-UvrD (75, 75, 1 nM). Panels below the gel show quantified amounts of linear dsDNA substrate (open circles) and nicked circular dsDNA product (closed squares).