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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Hypertens. 2013 Feb;31(2):303–311. doi: 10.1097/HJH.0b013e32835bbb83

Figure 1. Insulin activates the renal thiazide-sensitive Na+:Cl− cotransporter, NCC, in X. laevis oocytes and in mDCT15 cells.

Figure 1

A. Na+ uptake was determined in the absence (white bars) or presence of 20 U/ml insulin (black bars) in microinjected oocytes with water or with rNCC cRNA and in the absence or presence of metolazone, as stated. (p<0.001 vs. all other groups). B. The thiazide-sensitive Na+ uptake observed in rNCC control in the absence of insulin was taken as 1, and the effect of insulin was normalized accordingly. (N=22; *p<0.001 vs control). (C) NCC activity was analyzed in mDCT15 cells endogenously expressing NCC in the absence (white bar) or presence (black bar) of insulin. (N=5; *p<0.001).