Skip to main content
. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Hypertens. 2013 Feb;31(2):303–311. doi: 10.1097/HJH.0b013e32835bbb83

Figure 4. Effect of wild type and mutant WNK3 on the NCC basal activity and response to insulin.

Figure 4

Xenopus laevis oocytes were injected with rNCC cRNA alone or together with wild type WNK3 or the dominant negative WNK3-D294A, as stated. Three days later tracer Na+ uptake was assessed in the absence (white bar) or presence (black bar) of insulin. (A) The Na+ uptake observed in oocytes injected with rNCC alone in the absence of insulin was taken as 1 and all other groups were normalized accordingly. *p<0.01 vs rNCC control. (B) The Na+ uptake observed for each group in the absence of insulin was taken as 1 and in the presence of insulin was normalized to its own control group. *p<0.0001 to control group. #p<0.0001 vs. rNCC alone in the presence of insulin. Figures show compiled results from at least five different experiments of each group.