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. Author manuscript; available in PMC: 2013 Sep 24.
Published in final edited form as: Horm Metab Res. 2012 Sep 18;45(2):159–168. doi: 10.1055/s-0032-1321789

Fig. 3.

Fig. 3

mRNA and protein expression of selected NRs and HDAC11 in NSCs before and after FGF2 withdrawal. a, b, and c mRNA expression of selected NRs and HDAC11 in NSCs upon FGF2 withdrawal. mRNA expression of THRα, RORγ, COUP-TFI and ERRβ a, GR, MR, AR and PR b, and LRH1, PMC11, and HDAC11 c were examined with the SYBR Green-based real-time PCR using their specific primers listed in Table 1. Total RNA samples employed in Fig. 1 were used. Bars represent the mean ± S.E. values of fold mRNA expression upon FGF2 withdrawal compared with baseline (in the absence of FGF2 withdrawal). D: days after GFG2 withdrawal. *p < 0.05, **p < 0.01, n. s.: not significant, compared to baseline. d Protein expression of MR and HDAC11 in NSCs upon FGF2 withdrawal. NSCs before and after incubation for 5 days in the absence of FGF2 were immunostained with anti-MR or -HDAC11 antibody, and subsequently, by appropriate fluorescence-conjugated secondary antibodies. Cells were also stained with DAPI.