Table 2.
Ct values of NRs and coregulators in NSCs.
| Subfamilies | Genes | Name | Symbol | Mean | SE | |
|---|---|---|---|---|---|---|
| Nuclear Receptors | ||||||
| 1 | 1A | NR1A1 | Thyroid hormone receptor α | THRα | 25.41 | 0.379 |
| 2 | NR1A2 | Thyroid hormone receptor β | THRβ | 30.97 | 0.645 | |
| 3 | 1B | NR1B1 | Retinoic acid receptor α | RARα | 45.73 | 1.253 |
| 4 | NR1B2 | Retinoic acid receptor β | RARβ | 39.64 | 0.281 | |
| 5 | NR1B3 | Retinoic acid receptor γ | RARγ | 28.58 | 0.142 | |
| 6 | 1C | NR1C1 | Peroxisome proliferator-activated receptor α | PPARα | 30.33 | 0.171 |
| 7 | NR1C2 | Peroxisome proliferator-activated receptor γ | PPARδ | 25.88 | 0.040 | |
| 8 | NR1C3 | Peroxisome proliferator-activated receptor γ | PPARγ | 38.88 | 0.179 | |
| 9 | 1F | NR1F1 | RAR-related orphan receptor α | RORα | 26.28 | 0.126 |
| 10 | NR1F2 | RAR-related orphan receptor β | RORβ | 27.3 | 1.673 | |
| 11 | NR1F3 | RAR-related orphan receptor γ | RORγ | 34.3 | 0.195 | |
| 12 | 1H | NR1H2 | Liver X receptor β | LXRβ | 29.15 | 0.085 |
| 13 | NR1H3 | Liver X receptor α | LXRα | 29.75 | 0.065 | |
| 14 | NR1H4 | Farnesoid X receptor α | FXRα | 39.28 | 0.538 | |
| 15 | NR1H5 | Farnesoid X receptor β | FXRβ | 38.66 | 2.041 | |
| 16 | 1I | NR1I1 | Vitamin D receptor | VDR | 37.05 | 0.404 |
| 17 | NR1I2 | Pregnane X receptor | PXR | 31.16 | 0.220 | |
| 18 | NR1I3 | Constitutive androstane receptor | CAR | 25.46 | 0.081 | |
| 19 | 2A | NR2A1 | Hepatic nuclear receptor 4 α | HNF4α | 33.61 | 0.077 |
| 20 | NR2A2 | Hepatic nuclear receptor 4 γ | HNF4γ | 42.93 | 0.306 | |
| 21 | 2B | NR2B1 | Retinoid X receptor α | RXRα | 27.63 | 1.139 |
| 22 | NR2B2 | Retinoid X receptor β | RXRβ | 24.60 | 0.208 | |
| 23 | NR2B3 | Retinoid X receptor γ | RXRγ | 33.66 | 0.996 | |
| 24 | 2C | NR2C1 | Testicular receptor 2 | TR2 | 26.01 | 0.396 |
| 25 | NR2C2 | Testicular receptor 4 | TR4 | 22.41 | 0.008 | |
| 26 | 2E | NR2E2 | Tailless | TLX | 31.06 | 0.224 |
| 27 | NR3E3 | Photospecific nuclear receptor | PNR | 34.42 | 0.285 | |
| 28 | 2F | NR2F1 | Chicken ovalbumin upstream promoter-TFI | COUP-TFI | 26.85 | 1.530 |
| 29 | NR2F2 | Chicken ovalbumin upstream promoter-TFII | COUP-TFII | 26.37 | 0.053 | |
| 30 | NR2F6 | v-ErbA related 2 | EAR2 | 25.25 | 0.012 | |
| 31 | 3A | NR3A1 | Estrogen receptor α | ERα | 41.43 | 0.706 |
| 32 | NR3A2 | Estrogen receptor β | ERβ | 33.91 | 0.489 | |
| 33 | NR3B1 | Estrogen-related receptor α | ERRα | 42.7 | 2.114 | |
| 34 | 3B | NR3B2 | Estrogen-related receptor β | ERRβ | 32.31 | 0.126 |
| 35 | NR3B3 | Estrogen-related receptor γ | ERRγ | 38.99 | 0.396 | |
| 36 | 3C | NR3C1 | Glucocorticoid receptor | GR | 27.95 | 0.306 |
| 37 | NR3C2 | Mineralocorticoid receptor | MR | 37.07 | 0.130 | |
| 38 | NR3C3 | Progesterone receptor | PR | 30.58 | 0.097 | |
| 39 | NR3C4 | Androgen receptor | AR | 27.00 | 0.106 | |
| 40 | 4A | NR4A1 | NUR77 | NUR77 | 25.29 | 0.097 |
| 41 | NR4A2 | Nuclear receptor-related 1 | NURR1 | 27.78 | 0.073 | |
| 42 | NR4A3 | Neuron-derived orphan receptor 1 | NOR1 | 42.05 | 6.487 | |
| 43 | 5A | NR5A1 | Steroidogenic factor 1 | SF1 | 34.05 | 0.032 |
| 44 | NR5A2 | Liver receptor homologue 1 | LRH1 | 29.91 | 0.093 | |
| 45 | 6A | NR6A1 | Germ cell nuclear factor 1 | GCNF1 | 30.53 | 0.077 |
| 46 | 0B | NR0B1 | Dosage-sensitive sex reversal-adrenal hypoplasia congenita critical region on the X chromosome, gene 1 | DAX1 | 31.83 | 0.220 |
| 47 | NR0B2 | Small heterodimer partner | SHP | 37.48 | 0.167 | |
| Membrane nuclear receptors | ||||||
| 1 | Progesterone membrane component 11 | PMC11 | 26.44 | 0.024 | ||
| 2 | P Protein-coupled estrogen receptor 30 | GOCR30 | 39.69 | 1.686 | ||
| Coregulators | ||||||
| 1 | CREB-binding protein | CBP | 22.77 | 0.110 | ||
| 2 | p300 | p300 | 26.42 | 0.016 | ||
| 3 | Nuclear receptor coactivator 1 | NCoA1 | 25.21 | 0.016 | ||
| 4 | Nuclear receptor coactivator 2 | NCoA2 | 22.73 | 0.008 | ||
| 5 | Nuclear receptor coactivator 3 | NCoA3 | 24.61 | 0.073 | ||
| 6 | p300/CBP-associated factor | p/CAF | 28.45 | 0.318 | ||
| 7 | Nuclear receptor-interacting protein 1 | NRIP1 | 30.95 | 0.016 | ||
| 8 | PPARγ coactivator 1 α | PGC1α | 26.56 | 0.183 | ||
| 9 | Nuclear hormone receptor coregulator 6 | NRC6 | 32.37 | 0.044 | ||
| 10 | Structural maintenance of chromosome protein 1 (SMC) | SMC1 | 29.4 | 0.383 | ||
| 11 | Structural maintenance of chromosomes protein 4 | SMC4 | 29.54 | 0.457 | ||
| 12 | Thyroid receptor-associated proteincomplex 220 kDa component | TRAP220 | 23.07 | 0.089 | ||
| 13 | Thyroid hormone receptor-associated protein complex 170 kDa component | TRAP170 | 23.20 | 0.526 | ||
| 14 | Mediator of RNA polymerase II transcription subunit 6 | MED6 | 27.07 | 1.355 | ||
| 15 | Histone deacetylase 1 | HDAC1 | 22.35 | 0.024 | ||
| 16 | Histone deacetylase 2 | HDAC2 | 30.02 | 0.685 | ||
| 17 | Histone deacetylase 3 | HDAC3 | 22.01 | 0.061 | ||
| 18 | Histone deacetylase 4 | HDAC4 | 30.52 | 0.028 | ||
| 19 | Histone deacetylase 6 | HDAC6 | 25.27 | 0.044 | ||
| 20 | Histone deacetylase 7A | HDAC7 | 25.18 | 0.032 | ||
| 21 | Histone deacetylase 10 | HDAC10 | 31.39 | 0.269 | ||
| 22 | Histone deacetylase 11 | HDAC11 | 31.54 | 0.216 | ||
| 23 | Nuclear receptor corepressor 1 | NCoR1 | 25.56 | 0.069 | ||
| 24 | Nuclear receptor corepressor 2 | NCoR2 | 29.98 | 0.069 | ||
| 25 | SIN3 homolog | Sin3A | 24.64 | 0.469 | ||
| 26 | Set/Template activating factor 1β | Set/TAF-Iβ | 22.93 | 0.004 | ||
| 27 | Coactivator-associated arginine methyltransferase-1 | CARM1 | 21.82 | 0.032 | ||
| 28 | HRMT1-like 2 | HRMT-like 2 | 22.43 | 0.171 | ||
| 29 | Suppressor of variegation 3–9 homolog 1 | SUV39H1 | 32.01 | 1.481 | ||
| 30 | C-terminal tail-binding protein 1 | CtBP1 | 19.63 | 0.036 | ||
| 31 | Growth arrest-specific 5 | Gas5 | 26.27 | 0.881 | ||
| 32 | Steroid receptor RNA coactivator | SRA | 26.35 | 0.351 | ||
| 33 | SNF2 histone linker PHD RING helicase | SHPRH | 23.49 | 0.077 | ||
| 34 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 | SMARCA4 | 20.13 | 0.032 | ||
| 35 | Brx (PRKA13) Rho-type GEF | AKAP13 | 29.47 | 0.012 | ||
| Controls | ||||||
| 1 | GUSB | 23.53 | 0.0938 | |||
| 2 | HPT1 | 22.22 | 0.0163 | |||
| 3 | HSP1b | 16.92 | 0.232 | |||
| 4 | GAPDH | 16.97 | 0.191 | |||
| 5 | β-Actin | 15.25 | 0.1633 | |||
Unexpressed molecules (Ct > 35) are shown in grey