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. Author manuscript; available in PMC: 2014 Jul 16.
Published in final edited form as: Circulation. 2013 Jun 10;128(3):225–236. doi: 10.1161/CIRCULATIONAHA.112.000862

Figure 3.

Figure 3

MIF20 action in cardiomyocytes genetically deficient in MIF (MIF-KO) or the MIF cell surface receptor, CD74 (CD74-KO). Isolated mouse cardiomyocytes from the indicated mouse strains were incubated with MIF (8 nM) together with MIF20 (8 nM) or the MIF antagonist ISO-1 (1 μM) as shown. (A) The intracellular content of pAMPK (Thr172) and pACC (Ser79), and (B) cell surface GLUT4 levels in MIF-KO cardiomyocytes. (C) The intracellular content of pAMPK (Thr172) and pACC (Ser79), and (D) cell surface GLUT4 levels in MIF receptor (CD74-KO) cardiomyocytes. All values are means ± SEM for 3 independent experiments. *p<0.05 vs. corresponding control; †p<0.05 vs. MIF alone.