Table 1.
Twelve miRNAs are altered in end-stage ALS mouse and rat spinal cords by microarray
| Mouse |
Rat |
|||||
|---|---|---|---|---|---|---|
| RQ: SOD1G93A/non-TG | T-test | RQ: SOD1G93A/non-TG | T-test | RQ: SOD1G93A/SOD1WT | T-test | |
| miR-17 | 2.66 | 0.00 | 1.99 | 0.04 | 11.58 | 0.00 |
| miR-19b | 2.51 | 0.00 | 1.99 | 0.10 | 9.20 | 0.02 |
| miR-20a | 2.33 | 0.01 | 1.79 | 0.10 | 20.60 | 0.01 |
| miR-24-2-5p | 2.44 | 0.02 | 1.78 | 0.06 | 3.22 | 0.00 |
| miR-106a | 2.59 | 0.01 | 2.11 | 0.01 | 11.14 | 0.00 |
| miR-142-3p | 3.36 | 0.04 | 3.81 | 0.06 | 24.84 | 0.02 |
| miR-142-5p | 3.27 | 0.05 | 10.61 | 0.17 | 2.94 | 0.34 |
| miR-146a | 8.86 | 0.00 | 3.89 | 0.02 | 8.79 | 0.01 |
| miR-146b | 3.02 | 0.01 | 1.84 | 0.04 | 2.95 | 0.01 |
| miR-155 | 2.08 | 0.01 | 20.08 | 0.20 | 58.75 | 0.19 |
| miR-223 | 3.52 | 0.05 | 1.89 | 0.14 | 10.25 | 0.02 |
| miR-338-3p | 2.27 | 0.01 | 1.75 | 0.16 | 5.33 | 0.03 |
miRNAs were isolated from end-stage SOD1G93A mouse and rat spinal cords and from age-matched non-transgenic (non-TG) controls (n = 3–4). For rats, miRNAs were also isolated from age-matched SOD1WT control spinal cords (n = 3). A total of 673 miRNAs were assayed using Applied Biosystem's TaqMan Rodent Array A + B Cards v3.0 and 434 miRNAs amplified and were quantified. For mice, 6 miRNAs were decreased and 37 miRNAs were increased greater than 2-fold at P < 0.05. From these 43 miRNAs, 12 were also changed >50% in the rat samples at P < 0.20. RQ, relative quantification.