Figure 4.

A) CLISA test: wells of microtiter plates were coated with HSA samples and incubated in the following:

A: GCC labeled with mc-anti-HSA Ab including GCC-blocking solution

B: GCC solution including GCC-blocking solution (negative control)

C: GCC labeled with mc-anti-β-galactosidase Ab including GCC-blocking solution (negative control)

D: GCC labeled with mc-anti-HSA Ab, including GCC-blocking solution, incubated in blocked, washed, and without coated HSA-sample wells (negative control)

E: GCC labeled with pc-anti-BSA Ab including GCC-blocking solution (for testing the specificity of Ab)

F: incubation of GCC solution (without GCC-blocking solution) in wells coated with samples (to show the importance of blocking GCC particles using GCC-blocking solution)

Figure 4 B) ELISA test, incubated in:

A: wells of microtiter plates were coated with tris or HCl buffer and incubated in mc-anti-HSA Ab solution (negative control)

B: wells of microtiter plates were coated with HSA samples and incubated in mc-anti-HSA Ab solution.

Abbreviations: CLISA, cluster-linked immunosorbent assay; ELISA, enzyme-linked immunosorbent assay; HSA, human serum albumin.