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GCC flocculation can be avoided by increasing the amount of GCC solution, which was incubated with pc-antiperoxidase Ab to produce GCC-labeled Ab. A) GCC flocculation can be avoided by addition of protein G to the GCC solution before incubation with the Ab. B) No color reaction was observed by dotted β-LG protein (negative control).
Abbreviation: β-LG, β-lactoglobulin.
