Figure 4.

Quantification of aromatase expression in undifferentiated NSCs. NSCs isolated from the SVZ of three-month-old male and female rats and grown as neurospheres were then assessed for the level of expression of aromatase. A) The identification and quantification of aromatase expressed was then performed by western blot chemiluminescence using the NSCs of three different rats of each gender. Aromatase expression was also detected by immunofluorescence and confocal microscopy (B, C). Phase contrast (D, E).

Notes: Statistical analysis was conducted by Student’s t-test. *P < 0.05.

Abbreviation: ns, non significant; NSC, neural stem cell; SVZ, subventricular zone.