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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Gastroenterology. 2013 May 2;145(2):383–95.e1-21. doi: 10.1053/j.gastro.2013.04.050

Figure 7.

Figure 7

The new culture strategy enables highly efficient human crypt culture and maintenance. (A) Crypt culture strategy. CHIR99021 significantly increased the efficiency of culturing human small intestinal crypts. Data are presented as mean ± SEM (n = 6, *P .011, t test). (B) Representative growth process of human small intestinal crypt culture with addition of a single dose of GSK inhibitor CHIR99021 and other factors.33 (C) Human enteroid can be passaged up to 10 times over a 3-month period, as shown by their growth after passaging from day 0 to day 7. (D) IHC staining show the cellular proliferation and differentiated cell types in cultured enteroid. Positive staining is indicated by the arrow in the inset. Scale bars = 200 μm (B and C); 50 μm (D).