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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Am J Transplant. 2013 Jul;13(7):1891–1897. doi: 10.1111/ajt.12284

Figure 1.

Figure 1

Evaluation of urine RNA. Using reagents and samples prepared at site one, each laboratory isolated RNA from three urine pellet samples for qPCR analysis. A) Nanodrop absorbance spectra of 7 urine RNA samples isolated at site 1 illustrates typical appearance of urine RNA. B) Agilent Bioanalyzer electropherogram of a representative urine RNA indicates mostly small fragments of RNA; C) Nanodrop OD readings of the RNA isolated from urine pellet 1 in each site, showing normal yield and purity for urine samples; D) qPCR analysis of 3 urine pellet samples for 18S, and two samples for CXCL9, CXCL10, and IL8. All pre-analytical steps were performed at the respective sites using centrally provided reagents. Quantity and grand mean for each target and sample are plotted on a log scale. 18S sample 1 and CXCL10 samples 1 and 2 have one missing data point; all other samples show data from 5 sites.