Table 3.
Multivariate analysis of qPCR results from multiple sites
| A | blood | ||||
|---|---|---|---|---|---|
| site 1 | site 2 | site 3 | site 4 | site 5 | |
|
|
|||||
| site 1 | 1 | 0.9946 | 0.994 | 0.9794 | 0.9967 |
| site 2 | 0.9946 | 1 | 0.9978 | 0.982 | 0.9983 |
| site 3 | 0.994 | 0.9978 | 1 | 0.981 | 0.9984 |
| site 4 | 0.9794 | 0.982 | 0.981 | 1 | 0.9804 |
| site 5 | 0.9967 | 0.9983 | 0.9984 | 0.9804 | 1 |
| B | urine | ||||
|---|---|---|---|---|---|
| site 1 | site 2 | site 3 | site 4 | site 5 | |
|
|
|||||
| site 1 | 1 | 0.9983 | 0.9982 | 0.9913 | 0.9997 |
| site 2 | 0.9983 | 1 | 0.9971 | 0.9908 | 0.9984 |
| site 3 | 0.9982 | 0.9971 | 1 | 0.9929 | 0.9989 |
| site 4 | 0.9913 | 0.9908 | 0.9929 | 1 | 0.9911 |
| site 5 | 0.9997 | 0.9984 | 0.9989 | 0.9911 | 1 |
Expression of 18S, IL8, CXCL9, CXCL10, PRF1, and GZMB were measured in identical blood and urine cDNA samples at 5 clinical sites using different master mixes and PCR instruments Pearson correlations were calculated on CTs.