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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Pharm Sci. 2012 Jul 6;101(10):3763–3778. doi: 10.1002/jps.23256

Figure 2. Size distribution of antibody particles.

Figure 2

A. Comparison of unprocessed, lyophilized and dispersed 1B7 by DLS. All samples were diluted to 5 mg/ml in PBS. B. Effect of antibody concentration on particle size in dispersion buffer. At high concentration (200 mg/ml) in dispersion buffer, dynamic light scattering (DLS) detects only large particles of ~430 nm. Upon dilution below the threshold concentration for cluster formation in the dispersion buffer, concentrations of 2.5 and 1.25 mg/ml detect only particles of ~10 nm size, the expected size for monomeric IgG antibody. DLS data was analyzed by CONTIN.