sad-67 bypasses the spoIIG transcription defects of ymcA, ylbF and yaaT mutants. Luciferase assays for a PspoIIG-luc reporter were carried out as described in Experimental procedures. Strains were grown in the presence of 1 mM IPTG to induce expression of spo0A (A) or sad67 (B) from the Pspac promoter. The graphs are color coded as indicated. (A) Pspac-spo0A (BD6379), Pspac-spo0A ymcA (BD6385), Pspac-spo0A ylbF (BD6386), Pspac-spo0A yaaT (BD6387). (B) sad-67 (BD6380), sad-67 ymcA (BD6381), sad-67 ylbF (BD6382) and sad-67 yaaT (BD6383).