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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Mol Microbiol. 2013 Mar 12;88(2):283–300. doi: 10.1111/mmi.12186

Figure 6.

Figure 6

Inactivation of spo0E, but not of rapA or kinD bypasses the spoIIG transcription defects of ymcA, ylbF and yaaT mutants. Luciferase assays for expression from the PspoIIG-luc reporter construct were carried out as described in Experimental procedures. The strains carried knockouts of ymcA, ylbF or yaaT, as well as knockout mutations of rapA, kinD or spo0E. All yaaT strains were grown in the presence of 1 mM IPTG. The graphs are color coded as indicated in the figure panels. Strains included are as follows: Wild type (BD5966), ymcA (BD5669), ylbF (BD5670), yaaT (BD5671), rapAphrA (BD6213), ymcA rapAphrA (BD6214), ylbF rapAphrA (BD6215), yaaT rapAphrA (BD6216), kinD (BD6600), ymcA kinD (BD6601), ylbF kinD (BD6602), yaaT kinD (BD6603), spo0E (BD6219), ymcA spo0E (BD6220), ylbF spo0E (BD6221), and yaaT spo0E (BD6222).