Figure 3.

Phenotypic characterization of BamA barrel mutants. (A) Tenfold dilutions of stationary-phase cultures of the indicated mutants were spotted onto LB with or without 50 μg/ml erythromycin (Ery), 10 μg/ml rifampin (Rif), or 0.5% SDS + 1.0 mM EDTA and incubated at 37°. Column headings represent log concentrations relative to undiluted cultures. (B) Close-up of colonies from Figure 3A. Colonies formed by the indicated strains are shown following overnight growth on LB at 37°. The bamAR661G+D740G double mutant exhibits mucoidy under these conditions. (C) Levels of BamA and the major OMP LamB in exponential phase whole-cell extracts of the indicated strains were determined by SDS–PAGE and immunoblotting. The OM lipoprotein BamC, levels of which are not affected by OMP biogenesis defects, is shown as a control.