Skip to main content
. 2013 Oct;195(2):443–455. doi: 10.1534/genetics.113.155861

Figure 4.

Figure 4

BamA folding and stability in the presence of barrel mutations and suppressors. (A) Samples of the indicated strains were lysed gently and incubated at either 100° (+) or 24° (−) for 10 min prior to SDS–PAGE. Stably folded BamA (fBamA) migrates at a lower apparent molecular weight than the denatured protein (uBamA). (B) Whole-cell extracts were prepared using stationary-phase (overnight) cultures of the indicated strains. Samples were subjected to SDS–PAGE and immunoblotting for BamA and LamB.