Table 1. Effect of bamAD740G suppressor mutations on SDS-EDTA sensitivity.
| Parent allele | Intragenic secondary mutations and phenotypesa |
||||||
|---|---|---|---|---|---|---|---|
| POTRA5 |
β-barrel |
||||||
| Noneb | 395V | 423I | 607A | 631V | 631W | 718L | |
| bamAWT | R | R | R | R | R | R | R |
| bamAR661G | S | R | R | R | R | R | R |
| bamAD740G | S | R | R | R | R | R | R |
a
Phenotype refers to the growth of strains with the indicated genotypes on LB containing 0.5% SDS + 1.0 mM EDTA. Strains that exhibit growth after overnight incubation at 37° are considered resistant (R), and those that do not are considered sensitive (S).
b
No secondary mutation.