Figure 2.

Tor2 dysfunction causes pleiotropic phenotypes. (A) Genetic interaction between Tor2, Tsc2, and Npr2. The tor2-287 mutation caused pleiotropic phenotypes. The indicated single and double knockout cells were spotted onto the plates as indicated and then incubated for 3 days at 33° or 4–5 days at 27°. The cells were spotted in serial 10-fold dilutions starting with OD₆₆₀ = 0.3 of log-phase cells (5 μl). (B) The tor2⁺ gene rescued canavanine resistance of the tor2-287 mutants. The tor2-287 cells transformed with the control vector or the vector containing the tor2⁺ gene were streaked onto the plates as indicated and then incubated for 4–5 days at 27°. (C) The phenotypes of tor2 point mutation mutants. The indicated cells were spotted onto the indicated plates and incubated for 4–5 days at 27°.