Figure 1.

Integration profiling of S. pombe resulted in 360,513 independent insertions. (A) With integration profiling, genes required for cell division are predicted to have many fewer insertions than genes that are not required for propagation. (B) Transposition was induced in cells that contained one plasmid expressing the Hermes transposase and a second plasmid that contained the Hermes TIRs flanking kanMX6. Cells induced for transposition were propagated for many generations in serial cultures. (C) The positions of the integrations generated in vivo were plotted relative to ORFs. Each ORF was divided into 15 equal-sized segments and the number of insertions in each segment was displayed. Integration sites in intergenic regions closer to the 5′ end of an ORF were plotted upstream of the ORF in 100-bp intervals. The insertions closer to the 3′ end of an ORF were plotted downstream of the ORF. (D) The position of the insertions generated in vitro were plotted relative to ORFs as in C.