Figure 5.

Essential ORFs exhibited low integration densities. (A) The in vivo integration densities (inserts/kb/million integrations) for each ORF were determined and the distributions were plotted. (B) The distributions of in vivo integration densities were plotted for ORFs designated by the deletion consortium as essential (red) and nonessential (blue). The distribution of densities was also plotted for ORFs that when deleted, result in small colonies (green). (C) The in vitro integration densities (inserts/kb/million integrations) for each ORF were determined and the distributions were plotted as above in B.