Figure 3.
Cyclical epigenetic regulation of the MUC2 and RRM1 genes. (A) The kinetics of MeDIP, hMeDIP, and ChIP experiments were analyzed at the indicated time. The numbers of immunoprecipitated genes or methylated genes were quantified by qPCR and normalized to inputs according to the previous description. The number of generations (F0, F1, and F2) was determined by counting the cells after an initial synchronization in the G0/G1 phase (serum deprivation, 72 hours) (Suppl. Fig. S3). S phases (gray areas) were determined by measuring the 3H-thymidine incorporation in the DNA of U251 cells (Suppl. Fig. S3). (B) qPCR monitoring of expression levels of the MUC2 and RRM1 genes at certain times (as explained in A).