Figure 3.

Comparative analysis of growth of S100A4-positive and S100A4-negative human tumor cells in an athymic nude mouse model and quantification of extracellular S100A4 in culture media of CaP cells. (A, B) Representative photomicrographs show the visible differences between the S100A4-negative and S100A4-positive tumors implanted in athymic nude mice. (C) Graphical representation of data showing the growth of S100A4-negative and S100A4-positive tumors implanted in athymic mice. Data are represented as the mean ± SE. *P < 0.05 from the control group. (D) Line graph shows the number of mice reaching the preset end point of tumor volume of 1,000 mm³ in indicated weeks. (E) Histogram showing the proliferation of tumor cells in vivo (in terms of BrdU-positive cells/field). (F) Bands in the image represent the presence of the S100A4 protein in serum-free conditioned culture media of cells as assessed by a Slot-blot immunoreactivity assay. Recombinant S100A4 (dissolved in serum-free media) and PANC1 cells were used as a positive control. (G, H) Histogram showing levels of the S100A4 protein in the serum-free conditioned culture media of cells as measured by ELISA. Each bar in the histogram represents the mean ± SE. *P < 0.05. All experiments were repeated 3 times.