Figure 1. Phenotypic characteristics of p53-specific Tr1 cells generated in vitro. (A) Cytofluorometric analysis of Tr1 cells generated in the presence of autologous immature dendritic cells (iDCs) pulsed with various doses of a wild-type p53-derived (p53108–122) peptide. CD4+CD25− T cells cultured for 10 d in the presence of 150 IU/mL interleukin-2 and unpulsed iDCs served as control cells. Data are means ± SD (n = 5 experiments, performed with cells from different donors). (B) Phenotype of Tr1 cells generated in the presence of autologous iDCs pulsed with various doses of p53108–122. One representative experiment out of 5 performed with cells from different donors is shown.