Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Sep 23;210(10):1961–1976. doi: 10.1084/jem.20122508

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Acute deprivation of RA signaling alters splenic cDC composition without impacting subset proliferation or survival. Mice fed a normal (Ctrl) diet were injected (i.p.) daily for 10 d with a pan-RAR antagonist (220 µg BMS493) or DMSO vehicle Ctrl. Spleens from treated mice were then harvested and analyzed by flow cytometry by gating on live cDCs (CD11c^highI-A/E⁺). (A–C) Scatter plot showing the ratio of splenic CD11b⁺CD8α⁻/CD11b⁻CD8α⁺ cDCs (A) and bar graphs demonstrating the frequency of either annexin V surface (B) or Ki67 intranuclear staining (C) in gated cDC subsets from Ctrl diet mice treated either with BMS493 or DMSO; n = 4 mice per group. Results from one of two representative experiments are shown. Bars indicate means ± SEM of individually evaluated mice. **, P < 0.01 (unpaired Student’s t test).