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. 2013 Sep 23;210(10):1947–1960. doi: 10.1084/jem.20130512

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© 2013 Maruyama et al.

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Figure 6. — Osteoclast differentiation markers are normal in Sbno2^−/− osteoclasts. (A) MDMs from wild-type and Sbno2^−/− mice were cultured in the presence of 50 ng/ml RANKL. Cells were harvested in the indicated times, and TRAP, CTSK, calcitonin receptor, Jdp2, c-Fos, NFATc1, c-Src, CSK, CD44, Atp6v0d2, OC-STAMP, and β3-integrin levels were measured by qPCR. (B) qPCR analysis of Prdm1, Irf8, and Bcl6 levels in wild-type and Sbno2^−/− MDMs stimulated with 50 ng/ml RANKL for 84 h. (C) MDMs from wild-type and Sbno2^−/− mice were cultured in the presence of 50 ng/ml RANKL. Cells were harvested in the indicated times, and ephrin B2 and Sema4D levels were measured by qPCR. Results are representative of three (A and B) or two (C) independent experiments. Error bars, SE, n = 3.