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. 2013 Sep 23;210(10):2119–2134. doi: 10.1084/jem.20130252

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© 2013 Yang et al.

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Figure 4. — Silencing PFKFB3 constrains glucose metabolism in T cells. T cells were transfected with PFKFB3 shRNA plasmids (shPFKFB3-GFP) or control plasmids on day 2 after stimulation. Transfection efficiency was monitored by flow cytometry for GFP-expressing cells (A), and PFKFB3 transcripts were quantified 24 h later in control and shPFK-transfected cells by RT-PCR (B). Lactate production (C) and concentrations of intracellular ATP (D) were compared in five independent experiments, and results are given as mean ± SEM. Apoptotic cells were detected by flow cytometry; representative dot blots are shown (E). Expression of the lineage commitment transcription factors T-bet, Gata-3, FoxP3, and RORγt was measured in T cells 24 h after transfection (F), n = 8. *, P < 0.05; **, P < 0.01.