Figure 2. T-cell reactivity against AIM2 and NA17-A in the circulation of melanoma patients. (A, C, and E) T-cell responses measured with MHC multimers. Responses detectable on freshly thawed peripheral blood lymphocytes (PBLs) are illustrated in gray, while responses detectable upon culture in the presence of relevant peptides are shown in black. Striped bars indicate responses that were below the detection limit of 10 MHC multimer⁺ events. The X-axis report patient ID (MM), while the Y-axis (logarithmic scale) depicts the frequency of multimer⁺ cells. Representative dot plots are shown in Figure S1. (B, D, and F) T-cell responses measured with interferon γ (IFNγ)-specific ELISPOT assays. Black bars indicate positive responses, with more than twice as many spots in experimental wells than in control wells, while white bars indicate responses that were below the detection limit. Data are reported as mean ± SD of 2 replicate measurements. The X-axis report patient ID while the Y-axis depict the number of spots per 10⁵ cells recorded in IFNγ ELISPOT assays.