Table 1.

Dilution scheme used for the assay of conjugative action of SL and tetracycline against S. aureus.

Sr. no.	Test reaction description	Volume of SL stock (μL)	Volume of antibiotic stock (μL)	Volume of sterile distilled water (μL)	Volume of bacterial suspension (μL)	Total volume (μL)
1	Control	—	—	800	200	1000
2	SL alone	30	—	770	200	1000
3	SL + tetracycline	30	15	755	200	1000
4	Tetracycline	—	15	785	200	1000