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Recombinant YB-1 protein and lysates from tumor cells were immunostained with anti-YB-1 PAbs, mAbs 1-D9 and commercial YB-1 mAbs against YB-1. lane 1, recombinant YB-1; lane 2, breast cancer cell MDA-MB-231 lysates; lane 3, hepatic carcinoma cell HepG2 lysates; lane 4, non-small-cell lung cancer cell A549 lysates. Arrows indicated 50kDa YB-1.
