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. 2012 Jun 12;1(3):79–90. doi: 10.5501/wjv.v1.i3.79

Figure 4.

Figure 4

Long-term short hairpin RNA activity in human immunodeficiency virus type 1 replication studies. A: SupT1 T cells expressing short hairpin RNA (shRNA) Pol47 were infected with HIV-1 and virus replication was monitored in five cultures by measuring CA-p24 production for up to 75 d. Cells transduced with the empty lentiviral vector JS1 served as control (dark circles); B: Supernatant from the indicated culture (asterisk in panel A) was passaged on new cells to test the escape phenotype. The virus replicated on control and shRNA Pol47 cells, but not on cells that express another antiviral shRNA (R/T5) or the R4 construct. Adapted from[16].