Table 1.
Method | Advantages | Limitations |
Intracellular cytokine staining | Low volume blood | Flow cytometer required |
Rapid | Not standardized | |
Knowledge of HLA not required | ||
Identification of CD4+ and CD8+ | ||
MHC multimer staining | Low volume blood | Flow cytometer required |
Rapid | Not standardized | |
Elispot assay | Identification of CD4+ and CD8+ | Not standardized |
MHC: Major histocompatibility complex; HLA: Human leukocyte antigen.