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Culture with live cells is required to cause release of green fluorescence from DQ-substrates. Confocal images of green fluorescent channel at a z-level below the ECM-medium border. Cultures containing either collagen I with DQ-collagen I (A,B) or rBM containing DQ-collagen IV (C,D) were imaged after 3 days of culture without cells (A,C), with human breast cancer cells (B) or with human breast epithelial cells (D). Bar, 50 µm.
