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. 2013 Jul 8;2:148. [Version 1] doi: 10.12688/f1000research.2-148.v1

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Copyright: © 2013 Gilthorpe JD et al.

This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Data associated with the article are available under the terms of the Creative Commons Zero "No rights reserved" data waiver (CC0 1.0 Public domain dedication).

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Figure 2. — Adult conditioned medium (ACM) was made by incubating ischaemic brain slices in defined culture medium that was subjected to affinity chromatography. Western blots using antibodies specific to histones H1 and H2B show the presence of histone H1 at 32 kDa (lane 1) and histone H2B at 16 kDa in ACM (lane 3). Protein standards are shown at 32 kDa (H1 lane 2) and at 16 kDa for the monomer and 32 kDa for the dimer (H2B lane 4).