Figure 5. C. elegans growth in microchambers.

(A) Growth of a single C. elegans animal through the L1 larval stage constrained in a 200 x 200 x 18 µm polyacrylamide microchamber filled with OP50 as source of food. Time is shown in hours after hatching. At 12 h after hatching the animal has entered the lethargus at the end of the L1 larval stage. (B) Worm length as a function of time after hatching. Different colors indicate animals grown in parallel on the same device. Horizontal bars show the duration of lethargus, ending with the molt at the start of the L2 larval stage. The markers indicated by the arrow correspond to the time points shown in (A).