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. 2013 Sep 24;8(9):e75632. doi: 10.1371/journal.pone.0075632

Figure 2. dpn[a] and dpn[b] respond to Notch.

Figure 2

(A–B) Expression driven by dpn[a] or dpn[b] in third instar wing discs detected by immunostaining with anti-GFP (green) and anti-Dpn (blue). (A-A′′) Ectopic expression is detected when Notch intracellular domain (NICD) is expressed in the wing using patched-Gal4 (ptc>UAS-NICD) (position of ptc-expressing region indicated by white arrow). (B-B′′) Downregulation of Notch via RNAi using the same driver results in ablation of both endogenous (blue) and reporter (green) expressions at the D/V boundary (white arrow). (C-C′′) Ectopic expression of the NICD in R1, R3, R4, R6, and R7 with sev-Gal4 results in ectopic expression of endogenous Dpn (C′-red. Orange circle) and the GFP reporter (C′′-green. Orange circle and arrows) compared to wild type (D, blue circles and arrowheads).