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. 2013 Sep 24;8(9):e75490. doi: 10.1371/journal.pone.0075490

Figure 3. Growth and cytokine profile following L. monocytogenes infection in murine macrophages after simvastatin treatment.

Figure 3

(A) Murine BMDM and (B) RAW264.7 murine macrophage cell line were pretreated with the indicated concentrations of simvastatin, followed by L. monocytogenes infection (MOI=10). Bacterial growth was measured at 6 and 12 hours post-infection. (C) Macrophages were analyzed for statin-mediated cytotoxicity using MTT assay. Following simvastatin treatment and IFN-γ stimulation, macrophages were infected for 12 hours and supernatants were analyzed for the production of (D) IL-12p40, (E) TNF-α, (F) IL-6 and (G) nitric oxide. Results are shown as mean ± SEM of triplicate cultures and are representative of two independent experiments, * p < 0.05, ** p < 0.01 versus control.